10108014001
Roche
Papain
from Carica papaya
Sinónimos:
cystein protease, papain
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About This Item
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Productos recomendados
origen biológico
papaya
Nivel de calidad
Formulario
suspension
actividad específica
30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)
mol peso
23 kDa
envase
pkg of 10 mL (100 mg)
fabricante / nombre comercial
Roche
concentración
10-100 mg/mL
pH óptimo
6.0-7.0
Condiciones de envío
wet ice
Descripción general
Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.[1]
Aplicación
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.[4][5][6]
Acciones bioquímicas o fisiológicas
Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine.[1] It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.[2]
Características y beneficios
Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals
Forma física
Suspension, crystalline, nonsterile
Nota de preparación
Working concentration: 0.05 to 0.5 mg/ml
Almacenamiento y estabilidad
Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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