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Merck

10106399001

Roche

GTP

>90% (HPLC), crystals, pkg of 250 mg

Sinónimos:

Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na2

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About This Item

Fórmula empírica (notación de Hill):
C10H16N5O14P3 · xNa+ · yH2O
Número de CAS:
Peso molecular:
523.18 (anhydrous free acid basis)
Beilstein/REAXYS Number:
4113439
MDL number:
UNSPSC Code:
41106305
PubChem Substance ID:

description

Disodium salt

Quality Level

assay

>90% (HPLC)

form

crystals

mol wt

Mr 523.2 (GTP)
Mr 567.1 (GTP-Na2)

packaging

pkg of 250 mg

manufacturer/tradename

Roche

storage condition

avoid repeated freeze/thaw cycles

SMILES string

O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1

InChI key

HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

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General description

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
GTP is supplied as the disodium salt in crystal form.

Application

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Quality

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O

Preparation Note

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Ewa Król et al.
Journal of visualized experiments : JoVE, (81)(81), e50844-e50844 (2013-12-05)
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to form the so-called Z-ring. FtsZ polymerizes into long filaments in the presence of GTP in vitro, and polymerization is regulated by several accessory proteins.
Alice Cezanne et al.
eLife, 9 (2020-06-09)
Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation
The role of guanosine 5'-triphosphate in polypeptide chain elongation.
Y Kaziro
Biochimica et biophysica acta, 505(1), 95-127 (1978-09-21)
The role of guanosine 5'-triphosphate in the initiation of peptide synthesis. 3. Binding of formylmethionyl-tRNA to ribosomes.
T Ohta et al.
Proceedings of the National Academy of Sciences of the United States of America, 58(4), 1638-1644 (1967-10-01)
Lehninger, A.L
Biochemistry, 316-316 (1977)

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