MABS1118

Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1

clone 9D8.1, from mouse

• Sinónimos: F-box/WD repeat-containing protein 7, Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 9D8.1, monoclonal
• reactividad de especies: human, mouse
• técnicas: western blot: suitable
• isotipo: IgG1
• Nº de acceso NCBI: NP_361014
• Nº de acceso UniProt: Q969H0
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... FBXW7(55294)

Descripción

Descripción general

F-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NF B2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.

Especificidad

Clone 9D8.1 epitope lies within the N-terminal region specific to human and murine Cdc4 (FBXW7) spliced isoform 1 (Archipelago alpha, FBW7alpha, 110K, common), and not conserved in human/mouse isoform 2 (Archipelago beta, FBW7beta, 69K) or 3 (Archipelago gamma, FBW7gamma, Hippocampal). Note: UniProt Q8VBV4 currently lists only murine spliced isoform 3 (NM_080428), but not isoform 1 (NM_001177774) or 2 (NM_001177773).

Inmunógeno

GST-tagged recombinant human Cdc4 (FBXW7) isoform 1 (Archipelago alpha, FBW7alpha) N-terminal fragment.

Aplicación

Detect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cdc4 (FBXW7) in 10 µg of NIH/3T3, HeLa, HepG2, K562, LNCaP, U2OS nuclear extracts.

Calidad

Evaluated by Western Blotting in HEK293 nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc4 (FBXW7) in 10 µg of HEK293 nuclear extract.

Descripción de destino

~80 kDa observed. 79.66 kDa (human; UniProt Q969H0-1) and mouse; NP_001171244 & NM_001177774) calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable