MABE328

Anti-MeCP2 Antibody, clone 4H7

clone 4H7, from rat

• Sinónimos: Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4H7, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): rat (based on 100% sequence homology), porcine (based on 100% sequence homology)
• technique(s): ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_073164
• UniProt accession no.: Q00566
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Mecp2(17257)

Descripción

General description

DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

Specificity

This antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492.

Immunogen

Epitope: C-terminus

Full length, strep-tagged N-terminus of rat MeCP2.

Application

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This MeCP2 antibody is validated for use in WB, IHC, ICC, ChIP & IP for the detection of the MeCP2 protein

Quality

Evaluated by Western Blot in mouse brain tissue nuclear extract.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract.

Target description

~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue nuclear extract

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable