MABE1985

Anti-Gemin5 Antibody, clone 1E12

• Sinónimos: Gem-associated protein 5
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 1E12, monoclonal
• mol peso: calculated mol wt 168.59 kDa; observed mol wt ~170 kDa
• purificado por: using protein G
• reactividad de especies: human, human, monkey
• reactividad de especies (predicha por homología): porcine
• envase: antibody small pack of 100 μg
• técnicas: immunocytochemistry: suitable; western blot: suitable
• isotipo: IgG1
• secuencia del epítopo: C-terminal
• Nº de acceso Protein ID: NP_001239085
• Nº de acceso UniProt: Q8TEQ6
• Condiciones de envío: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GEMIN5(25929)

Descripción

Descripción general

Gem-associated protein 5 (UniProt: Q8TEQ6; also known as Gemin5) is encoded by the GEMIN5 gene (Gene ID: 25929) in human. Survival of motor neurons (SMN) protein is known to form a stable complex with a group of proteins named gemins. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs) and thereby plays an important role in the splicing of cellular pre-mRNAs. Gemin5, a highly conserved, multi-functional protein, is a component of the core SMN complex and is found in the nucleoplasm and nuclear bodies known as Gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. It is also abundantly present in the cytoplasm. It plays a role in capturing snRNA into SMN complexes in the cytoplasm for transport into the nucleus. However, in the nucleus it is easily detached from the SMN-Gemin complex. Gemin5 is reported to physically bind to snRNA via a specific AU5-6 sequence located within the highly conserved Sm site and is flanked by a short stem loop that assists in delivery to the SMN complex. The specific snRNP code helps Gemin5 in distinguishing these snRNAs from other forms of cellular RNAs. Gemin5 contains 13 WD repeat domains that mediate its binding to U1 and U4 snRNA. WD domains are also reported to mediate binding to the 7-methylguanosine cap that is found on mRNA and snRNA molecules. Mutations in GEMIN5 gene are known to cause a distinct neurological cerebellar ataxia syndrome, through altered snRNP complex assembly. (Ref.: Kour, S., et al. (2021). Nat. Commun. 12; Article 2558; Hao, L., et al. (2007). BMC Cell Biol. 8:28).

Especificidad

Clone 1E12 is a mouse monoclonal antibody that detects Gem-associated protein 5 (Gemin5). It targets an epitope within the C-terminal region.

Inmunógeno

His-tagged recombinant fragment corresponding to 292 amino acids form the C-terminal region of human Gem-associated protein 5 (Gemin5).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in COS-7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in COS-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in HeLa cell lysate.

Immunocytochemistry Analysis: A representative lot detected Gemin5 in Immunocytochemistry application (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28; Schilling, M., et al. (2021). Cell Rep. 35(12); 109277).

Western Blotting Analysis: A representative lot detected Gemin5 in Western Blotting applications (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable