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Key Documents

MABC290

Sigma-Aldrich

Anti-PDL-1/CD274 Antibody, clone 3F2.1

clone 3F2.1, 1 mg/mL, from mouse

Sinónimos:

Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, PDL-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F2.1, monoclonal

species reactivity

human

concentration

1 mg/mL

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD274(29126)

General description

Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.

Immunogen

Epitope: extracellular domain
GST-tagged recombinant protein corresponding to the extracellular domain of human PDL-1/CD274.

Application

Anti-PDL-1/CD274 Antibody, clone 3F2.1 is an antibody against PDL-1/CD274 for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected PDL-1/CD274 in human tonsil and skeletal muscle tissue, respectively.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in human skeletal muscle tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1/CD274 in 10 µg of human skeletal muscle tissue.

Target description

~40 kDa observed. The calculated molecular weight is 33 kDa; however, this protein may be observed at higher molecular weights to due to heavy glycosylation.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Visite la Librería de documentos

Programmed cell death ligand 1 as a biomarker in head and neck cancer.
Sara I Pai et al.
Cancer cytopathology, 125(7), 529-533 (2017-05-05)
Grégoire D'Andréa et al.
Theranostics, 11(3), 1310-1325 (2021-01-05)
The programmed cell death-1/programmed cell death ligand-1 (PD-1/PD-L1) immune checkpoint proteins hold promise as diagnostic, prognostic, and therapeutic targets for precision oncology. By restoring antitumor T cell surveillance, the high degree of effectiveness of the immune checkpoint inhibitors (ICIs) has

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