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Key Documents

MAB5424

Sigma-Aldrich

Anti-Prion Protein Antibody

Chemicon®, from mouse

Sinónimos:

PrP, CD230

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

sheep, mouse, mule deer, bovine, elk

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PRNP(5621)

Specificity

Reacts with prion protein (PrP) from bovine, sheep, mule deer, elk and mouse. The monoclonal recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).

Immunogen

Recombinant PrP amino acids 23-237.

Application

Anti-Prion Protein Antibody is an antibody against Prion Protein for use in ELISA, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:50,000-1:200,000

Immunohistochemistry: 1:100-1:500. Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:

a. formic acid for 10 minutes at room temperature

b. hydrolytic autoclaving

c. heat induced epitope retrieval

ELISA: 1:20,000-1:50,000

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS. Contains no preservative.

Storage and Stability

Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt.

Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ranjit Kumar Giri
Journal of translational medicine, 22(1), 503-503 (2024-05-28)
Prion diseases are transmissible and fatal neurodegenerative diseases characterized by accumulation of misfolded prion protein isoform (PrPSc), astrocytosis, microgliosis, spongiosis, and neurodegeneration. Elevated levels of cell membrane associated PrPSc protein and inflammatory cytokines hint towards the activation of death receptor
Juan Manuel Ribes et al.
Nature communications, 14(1), 8354-8354 (2023-12-16)
The self-templating nature of prions plays a central role in prion pathogenesis and is associated with infectivity and transmissibility. Since propagation of proteopathic seeds has now been acknowledged a principal pathogenic process in many types of dementia, more insight into

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