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Key Documents

MAB4133

Sigma-Aldrich

Anti-p16 Antibody, clone D25

clone D25, Chemicon®, from mouse

Sinónimos:

INK4a, MTS1, CDK4IN, CDKN2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

D25, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDKN2A(1029)

Specificity

P16* is a major tumor suppressor gene, which is known to be deleted or mutated in many types of human cancers. Antibody MAB4133 binds specifically to p16 in its recombinant or native form. It also recognizes p16 expressed by melanomas and neve in situ.

Immunogen

Recombinant p16 fusion protein from E. coli.

Application

Anti-p16 Antibody, clone D25 is a Mouse Monoclonal Antibody for detection of p16 also known as INK4a, MTS1, CDK4IN, CDKN2 & has been validated in WB & IHC.
Immunoblotting

Immunohistochemistry

Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Linkage

Replaces: 04-239

Physical form

Format: Purified
Liquid in phosphate buffer, containing azide as a preservative.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Analysis Note

Control
POSITIVE CONTROL: HeLa cells

colon carcinoma

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Matt Lechner et al.
Oral oncology, 83, 32-37 (2018-08-14)
p16INK4A (p16) is the most widely used clinical biomarker for Human Papillomavirus (HPV) in head and neck squamous cell cancer (HNSCC). HPV is a favourable prognostic marker in HNSCC and is used for patient stratification. While p16 is a relatively
Daniel J Asby et al.
Cancer management and research, 10, 3483-3500 (2018-09-27)
Diffuse intrinsic pontine glioma (DIPG) is a lethal type of pediatric brain tumor that is resistant to conventional chemotherapies. Palbociclib is a putative novel DIPG treatment that restricts the proliferation of rapidly dividing cancer cells via selective inhibition of cyclin-dependent
Hung-Chun Fu et al.
International journal of molecular sciences, 19(9) (2018-08-29)
Previous studies have suggested that cancer stem cells (CSCs) resisted radiotherapy and chemotherapy. P16INK4A is a biomarker for cervical carcinogenesis and reduces proliferation of stem cells. We aimed to investigate the expression and clinical significance of cyclin-dependent kinase inhibitor 2A
N Zhao et al.
British journal of cancer, 107(3), 482-490 (2012-06-28)
Recently, the management of head and neck squamous cell carcinoma (HNSCC) has focused considerable attention on biomarkers, which may influence outcomes. Tests for human papilloma infection, including direct assessment of the virus as well as an associated tumour suppressor gene
Alhadi Almangush et al.
British journal of cancer, 117(6), 856-866 (2017-07-29)
Identifying informative prognostic biomarkers for oral tongue squamous cell carcinoma (OTSCC) is of great importance in order to better predict tumour behaviour and to guide treatment planning. Here, we summarise existing evidence regarding immunohistochemical prognostic biomarkers for OTSCC. A systematic

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