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MAB2514

Sigma-Aldrich

Anti-Integrin αVβ1 Antibody

CHEMICON®, rat monoclonal, BMB5

Sinónimos:

VLA-5

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nombre del producto

Anti-Integrin α5β1 Antibody, clone BMB5, ascites fluid, clone BMB5, Chemicon®

origen biológico

rat

Nivel de calidad

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

BMB5, monoclonal

reactividad de especies

mouse

fabricante / nombre comercial

Chemicon®

técnicas

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG3

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ITGA5(3678)

Especificidad

Antibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

Inmunógeno

The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.

Aplicación

Inhibition of cell adhesion to fibronectin: 10 μg/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg

Flow cytometry: 10μg/mL

Western blot (non-denaturing gel)

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.

Forma física

Liquid, containing no preservatives.

Almacenamiento y estabilidad

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mediators of inflammation, 2016, 6120509-6120509 (2016-04-02)
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Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells.
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Scientific Reports null
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Reproductive biology and endocrinology : RB&E, 21(1), 85-85 (2023-09-16)
Adhesion of the implanting blastocyst involves the interaction between integrin proteins expressed by trophoblast cells and components present in the basement membrane of the endometrial luminal epithelium. Although several factors regulating integrins and their adhesion to fibronectin are already known
Nayeli Alva-Murillo et al.
Frontiers in cellular and infection microbiology, 7, 78-78 (2017-04-01)
Bovine mammary epithelial cells (bMECs) contribute to mammary gland defense against invading pathogens, such as Staphylococcus aureus (intracellular facultative), which is recognized by TLR2. In a previous report, we showed that sodium octanoate [NaO, a medium chain fatty acid (C8)]

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