HTS076RTA
Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells
Human
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.84
Productos recomendados
Nombre del producto
Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, Human PAF GPCR frozen cells for Calcium Flux FLIPR Assays.
origen biológico
human
Nivel de calidad
fabricante / nombre comercial
Ready-to-Assay
técnicas
calcium flux assay: suitable
Nº de acceso NCBI
método de detección
fluorometric
Condiciones de envío
dry ice
Descripción general
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.
Platelet-activating factor (PAF) is a bioactive phospholipid that was originally characterized by its ability to induce platelet aggregation, but was subsequently shown to mediate angiogenesis, inflammation and neural development. The PAF receptor is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and cytokine and leukotriene release (Stafforini et al., 2003). Millipore’s cloned human PAF-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PAF expression on the cell surface and contains high levels of the promiscuous G protein G15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PAF and its ligands.
Descripción línea celular
Host cells: Chem-1
Acciones bioquímicas o fisiológicas
Protein Target: PAF
Target Sub-Family: Platelet Activating Factor
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico