ABT98

Anti-Kinectin Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Kinectin, CG-1 antigen, Kinesin receptor
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): rhesus macaque (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_001072989
• Nº de acceso UniProt: Q86UP2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... KTN1(3895)

Descripción

Descripción general

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

Especificidad

Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

This antibody recognizes the lumenal region of Kinectin.

Inmunógeno

Epitope: Lumenal region

KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

Aplicación

Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Calidad

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

Descripción de destino

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human testis tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable