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AB9074

Sigma-Aldrich

Anti-IP3 Receptor 2 Antibody

serum, Chemicon®

Sinónimos:

Inositol 1,4,5-Trisphosphate Receptor 2

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

serum

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

human, mouse

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ITPR2(3709)

Especificidad

IP3 Receptor 2.

Inmunógeno

Synthetic peptide from the C terminus of human IP3 Receptor 2.

Aplicación

Detect IP3 Receptor 2 using this Anti-IP3 Receptor 2 Antibody validated for use in IC, IH & WB.
Research Category
Signaling
Research Sub Category
Lipid Signaling
Western blot: 1:1,000 using ECL. The antibody reacts with the 250 kDa IP3 Receptor 2 protein in microsomal preparations. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel or 5% PAGE. For microsomal methods and blotting procedure similar antibodies can be found in: Perez, et al. J Biol. Chem. 272:23961

http://www.jbc.org/cgi/reprint/272/38/23961?ijkey=35bcf508a3025fc8bf8203ea3805122eec0caf1e )

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Forma física

Rabbit serum. Liquid.

Almacenamiento y estabilidad

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Nota de análisis

Control
Western blot = adult mouse liver.

IHC = adult mouse hepatocytes (adult mouse cerebellum Purkinje cells will be negative).

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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