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Merck
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Key Documents

AB2148P

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody

Chemicon®, from rabbit

Sinónimos:

TNF-alpha

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

NCBI accession no.

NP_038721

UniProt accession no.

P06804

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TNF(7124)

Specificity

Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells.

Immunogen

Highly pure (>98%) recombinant Mouse TNF-alpha.

Application

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB.
ELISA: A concentration of at least 0.5μg/mL (100μL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha.

Western Blot: A concentration of 0.1-0.2 μg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 μg/ml of AB2148P is required.

Optimal working dilutions must be determined by the end user

Linkage

Replaces: AB1450P

Physical form

Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Although receptor-activator of nuclear factor κB (RANK), its ligand RANKL, and osteoprotegerin (OPG), which are members of the tumor necrosis factor (TNF) superfamily, were first discovered in bone cells, they are also expressed in other cells, including skeletal muscle. We
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