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Merck
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Key Documents

1.15684

Supelco

TLC plates, Silica gel 60 RP-8 F₂₅₄s

pkg of 25 plates, plate L × W 5 cm × 10 cm, glass support

Sinónimos:

RP-8 Silica Gel Thin-layer Chromatography Plates

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About This Item

UNSPSC Code:
41115711
NACRES:
NB.21

material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 25 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

250 μm

plate L × W

5 cm × 10 cm

particle size

10-12 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

25 Glass plates 5 x 10 cm
RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Application

Glass TLC plates, RP-8 modified silica gel coated with flourescent indicator F254s. The RP-8 modified silica gel TLC plates come in a quantity of 25 size 5x10 cm for thin layer chromatography applications.

Linkage

Replaces: 15684-1; 15684

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 9.5 - 11.5 µm
Layer thickness: 200 - 270 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
68 - 74
B) Steroid test
- methyltestosterone, steroid test: 45 - 55
- Reichstein S, steroid test: 65 - 75
- hydrocortisone, steroid test: 75 - 91
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)

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