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Key Documents

1.03871

Millipore

GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar

acc. SCHIEMANN, ISO 10273

Sinónimos:

CIN Agar, Cefsulodin, Irgasan, Novobiocin Agar, SCHIEMANN’S Agar, Yersinia Selective Agar (YSA)

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

agency

ISO 10273

Quality Level

form

powder

technique(s)

microbiological culture: suitable

pH

7.4±0.2 (25 °C)

application(s)

food and beverages
pharmaceutical

storage temp.

15-25°C

suitability

selective for (Microorganism: Yersinia)

General description

GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar is a nutrient rich selective medium which is designed for the isolation and differentiation of Yersinia enterocolitica from the food chain and other materials.
The selectivity of the medium is contributed using antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin, bile salts and crystal violet dye which synergistically inhibit the accompanying flora. The pyruvate in the medium promotes the growth of Yersinia spp. agar is the solidifying agent.
Mannitol from the medium is degraded by the growing Yersinia to form an acid which will trigger a pH change indicated by neutral red. The positive colonies appear red in color. The fermentation reaction by the growing Yersinia is intense and localized which produces the characteristic “bull′s eye” appearance. The microbes which do not metabolize mannitol from the media will form colorless, translucent colonies.

Application

GranuCult® prime CIN (Cefsulodin, Irgasan, Novobiocin) Agar is for the isolation and differentiation of Yersinia enterocolitica from the food chain and other materials.

Analysis Note

  • The antibiotics- Cefsulodin, Irgasan(Triclosan), Novobiocin are added separately after autoclaving.
  • The dehydrated medium is granulate with pink color.
  • The prepared medium is clear to slightly opalescent and red.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

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Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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