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Merck
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Key Documents

07-452

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Sinónimos:

H3K27me2, Histone H3 (di methyl K27)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003493

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

dimethylation (Lys27)

Gene Information

human ... H3C1(8350)

General description

Anti-dimethyl-Histone H3 (Lys27)

Specificity

Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Application

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Quality

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Target description

17 kDa

Physical form

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified

Storage and Stability

Stable for 2 years at 2-8°C from date of shipment.

Analysis Note

Control
HeLa whole cell lysate, U2OS cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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