05-1308
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal
clone Apu3, from rabbit
Sinónimos:
Anti-Ubiquitin, Clone Apu3 Anti-Ubiquitin, Lys63-Specific Ubiquitin Antibody
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
Apu3, monoclonal
reactividad de especies
rat, human, mouse, rabbit
técnicas
cell based assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... UBAP2(55833)
Descripción general
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
Especificidad
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Inmunógeno
Epitope: Lys63 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys63.
Aplicación
Immmunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates
Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates
Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin and has been validated in Inhibition Assay (PIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC) and Western Blotting (WB).
Research Category
Signaling
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
Calidad
Western Blot Analysis:
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
Descripción de destino
varies
Forma física
Format: Purified
Protein A purified
Protein A purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Almacenamiento y estabilidad
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Nota de análisis
Control
Lys63 polyubiquitin chains.
Lys63 polyubiquitin chains.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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