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Key Documents

04-1479

Sigma-Aldrich

Anti-methyl-PP2A Antibody, C subunit, clone 2A10

clone 2A10, from mouse

Sinónimos:

Anti-NEDLBA, Anti-PP2Ac, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2A10, monoclonal

species reactivity

yeast, human, Saccharomyces cerevisiae, mouse

species reactivity (predicted by homology)

rat, rabbit, chicken, pig, Drosophila

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

methylation (Leu309)

Gene Information

human ... PPP2CA(5515)

General description

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

Specificity

Recognizes the methylated form of PP2A, catalytic subunit. Exhibits some reactivity with methyl-PP4 and slight reactivity with methyl-PP6.

Immunogen

KLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.

Application

Research Category
Signaling
Research Sub Category
Transcription Factors
This Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A. Exhibits some reactivity with methyl-PP4 and slight reactivity with methyl-PP6.

Quality

Evaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.

Target description

~36 kDa

Linkage

Replaces: 05-546

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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