40718

Dietilpirocarbonato

96% (NT)

• Sinónimos: DEP, DEPC, Dietildicarbonato, Dietiloxidiformato, Ácido etoxifórmico anhidro
• Fórmula lineal: O(COOC₂H₅)₂
• Número de CAS: 1609-47-8
• Peso molecular: 162.14
• Beilstein/REAXYS Number: 637031
• EC Number: 216-542-8
• MDL number: MFCD00009106
• UNSPSC Code: 12352100
• PubChem Substance ID: 24865430
• NACRES: NA.22

Propiedades

• assay: 96% (NT)
• form: liquid
• color: APHA: ≤20
• refractive index: n20/D 1.398 (lit.); n20/D 1.398
• bp: 93-94 °C/18 mmHg (lit.)
• solubility: 95% ethanol: 4.5 g/10 mL, clear, colorless
• density: 1.121 g/mL at 20 °C; 1.101 g/mL at 25 °C (lit.)
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: CCOC(=O)OC(=O)OCC
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N

Descripción

General description

Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO₂ and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.

Application

Diethyl pyrocarbonate may be used in the following studies:

• For the modification of histidyl residues in proteins.
• As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
• For the modification of linear and supercoiled plasmid DNAs.
• As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
• For cabethoxylation of histidine residues of actin.

Reactivo de modificación para residuos de His y Tyr en proteínas. Sonda robusta para alteraciones estructurales en el ADN bicatenario, que reacciona con las bases total o parcialmente desapiladas.

Biochem/physiol Actions

Inactiva la ARNasa en disolución aproximadamente al 0,1% (v/v). Por tanto, protege el ARN contra la degradación.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: 156.2 °F - closed cup
• flash_point_c: 69 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter