SRP6273
UPA human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
ATF, BDPLT5, PLAU, QPD, UPA, URK, Urokinase, u-PA
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 45 kDa
observed mol wt 18 kDa
observed mol wt 32 kDa
observed mol wt 50 kDa
packaging
pkg of 10 μg
pkg of 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... uPA(5328)
General description
DTT-reduced. Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain due to glycosylation and cleavage. Ser21, Ile179 & Lys156 is the predicted N-terminus.
The PLAU (plasminogen activator, urokinase) gene is mapped to human chromosome 10q22.2.
Urokinase-type plasminogen activator is also known as PLAU and uPA. The human uPA is initially synthesized as 431 amino acid precursor with an N-terminal signal peptide (20 residues). The single chain molecule is processed into a disulfide-linked two-chain molecule of different molecular weights. Two forms of the A chain exist, starting at Ser21 (the long form) and Lys156 (the short form). The long and short A chains are unique to the high and low molecular weight forms, respectively. The long A chain contains an EGF (epidermal growth factor)-like domain, responsible for binding of the PLAU receptor. The B chain corresponds to the catalytic domain. The gene encoding it is localized on human chromosome 10.
Biochem/physiol Actions
Urokinase-type plasminogen activator is a serine protease with extremely limited substrate specificity, cleaving the sequence Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys in plasminogen to form plasmin. It is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium. uPA (plasminogen activator, urokinase) - mediated degradation of extracellular matrix of migrating cells is essential for tissue remodeling, invasiveness and angiogenesis. uPA promotes pathogenesis of Psoriasis and basal cell carcinoma. uPA activates growth factors and metalloproteinases.
Physical form
Lyophilized from 0.22 μm filtered solution in HEPES, NaCl and CaCl2. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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