T2824

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-TNF-α
• Numéro MDL: MFCD01632994
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: hamster
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TN3-19.12, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: rat, mouse
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~2 mg/mL
• Technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α
• Numéro d'accès UniProt: P16599
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Tnf(21926) ; rat ... Tnf(24835)

Description

Description générale

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.

Spécificité

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.

Immunogène

purified recombinant mouse TNF-α.

Application

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• neutralization

Actions biochimiques/physiologiques

TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

Forme physique

Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable