T2315

Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonyme(s) : CD120b antigen, Etanercept, TNF sRII, TNF-R2, p75 TNF receptor, p75TBPII, p75TNFR, tumor necrosis factor receptor superfamily member 1B
• Numéro MDL: MFCD01633483
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: goat
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: lyophilized powder
• Espèces réactives: human
• Technique(s): immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs); western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions)
• Numéro d'accès UniProt: P20333
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TNFRSF1B(7133)

Description

Description générale

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Spécificité

The antibody detects human TNF sRII and shows no cross-reactivity with recombinant human TNF sRI.

Immunogène

recombinant human TNF soluble receptor II (TNF sRII) expressed in Escherichia coli. The immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain.

Application

Western Blot: 1 ug/mL detects rhs TNF RII at 5 ng/lane under non-reducing conditions
Immunocytochemistry: Concentration of 5-15 ug/ml detects TNF RII in human peripheral blood lymphocytes

Optimal dilutions should be determined by the end user.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) and 5% trehalose.

Autres remarques

Protein A or G purified

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable