T111080

DMT-dT phosphoramidite

configured for PerkinElmer, configured for Polygen

• Synonyme(s) : 5′-O-(4,4′-diméthoxytrityl)-2′-désoxythymidine-3′-O-[O-(2-cyanoéthyl)-N,N′-diisopropylphosphoramidite], 5′-O-[bis(4-méthoxyphényl)phénylméthyl]-2′-désoxythymidine, 3′-[2-cyanoéthyl-N,N-bis(1-méthyléthyl)phosphoramidite], DMT-dT amidite, Thymidine, 5′-O-[bis(4-méthoxyphényl)phénylméthyl]-, 3′-[2-cyanoéthyl-N,N-bis(1-méthyléthyl)phosphoramidite]
• Formule empirique (notation de Hill): C₄₀H₄₉N₄O₈P
• Numéro CAS: 98796-51-1
• Poids moléculaire : 744.81
• Numéro MDL: MFCD00055063
• Code UNSPSC : 41116107
• ID de substance PubChem : 329826649
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: non-animal source (no BSE/TSE risk)
• Type: for DNA synthesis
• Gamme de produits: Proligo Reagents
• Pureté: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Forme: powder or granules
• Technique(s): oligo synthesis: suitable
• Impuretés: ≤0.3 wt. % water content (Karl Fischer)
• Couleur: white to off-white
• λ: conforms (UV/VIS Identity)
• Compatibilité: configured for PerkinElmer; configured for Polygen
• Profil des nucléosides: base: deoxythymidine; base protecting group: none; 2' protecting group: none; 5' protecting group: DMT; deprotection: fast/standard
• Température de stockage: 2-8°C
• Chaîne SMILES: COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C40H49N4O8P/c1-27(2)44(28(3)4)53(50-23-11-22-41)52-35-24-37(43-25-29(5)38(45)42-39(43)46)51-36(35)26-49-40(30-12-9-8-10-13-30,31-14-18-33(47-6)19-15-31)32-16-20-34(48-7)21-17-32/h8-10,12-21,25,27-28,35-37H,11,23-24,26H2,1-7H3,(H,42,45,46)/t35-,36+,37+,53?/m0/s1
• Clé InChI: UNOTXUFIWPRZJX-CEXSRUIHSA-N

Description

Description générale

Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)). Recommended cleavage and deprotection conditions are 8 hours at 55 °C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides. DMT-dT Phosphoramidite is configured for Expedite^™ and PolyGen^® Synthesizers.

Informations légales

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

PolyGen is a registered trademark of PolyGen GmbH.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable