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T1032

Sigma-Aldrich

TNM-FH insect medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

Synonyme(s) :

Graces Modified Medium

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About This Item

Numéro MDL:
MFCD00243216
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

500

Forme

powder

Concentration

50.6 g/L

Technique(s)

cell culture | insect: suitable

Adéquation

suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)

Composants

L-glutamine: 0.6 g/L
NaHCO3: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Catégories apparentées

Description générale

A number of modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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S E Crawford et al.
Journal of virology, 68(9), 5945-5952 (1994-09-01)
Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression
W J Shen et al.
The Journal of biological chemistry, 276(52), 49443-49448 (2001-10-30)
Hormone-sensitive lipase (HSL) is an intracellular lipase that plays an important role in the hydrolysis of triacylglycerol in adipose tissue. HSL has been shown to interact with adipocyte lipid-binding protein (ALBP), a member of the family of intracellular lipid-binding proteins
The baculovirus expression system: a laboratory guide.
King L
Springer Science & Business Media, 2012 null
M Schmidt et al.
The Journal of biological chemistry, 274(21), 14648-14654 (1999-05-18)
Stimulation of phospholipase D (PLD) by membrane receptors is now recognized as a major signal transduction pathway involved in diverse cellular functions. Rho proteins control receptor signaling to PLD, and these GTPases have been shown to directly stimulate purified recombinant
T T Chuang et al.
The Journal of biological chemistry, 271(45), 28691-28696 (1996-11-08)
G protein-coupled receptor kinases (GRKs) are implicated in the homologous desensitization of G protein-coupled receptors. Six GRK subtypes have so far been identified, named GRK1 to GRK6. The functional state of the GRKs can be actively regulated in different ways.
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