SAE0094
SAvPhire™ Monomeric Streptavidin
recombinant, expressed in E. coli, High Affinity
Synonyme(s) :
mSA3
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Forme
lyophilized powder
Conditions d'expédition
wet ice
Température de stockage
−20°C
Actions biochimiques/physiologiques
SAvPhire™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.
The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
- Minimizing distribution or dynamics in the probing of biotinylated targets
- Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
- Easier access to desired target
This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.
Propriétés physiques
Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag
Reconstitution
Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.
This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:
The reconstituted product can be further diluted in water or PBS as needed.
- 1 mg vial: add 250 μL of water for a 4 mg/mL solution
- 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution
The reconstituted product can be further diluted in water or PBS as needed.
Informations légales
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
SAvPhire is a trademark of Sigma-Aldrich Co. LLC
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Expression and purification of soluble monomeric streptavidin in Escherichia coli
Applied Microbiology and Biotechnology, 98(14), 6285-6295 (2014)
Functional expression of monomeric streptavidin and fusion proteins in Escherichia coli: applications in flow cytometry and ELISA.
Applied Microbiology and Biotechnology, 102, 10079-10089 (2018)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique