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SAB4300123

Sigma-Aldrich

Anti-phospho-JAK1 (pTyr1022) antibody produced in rabbit

affinity isolated antibody

Synonyme(s) :

Anti-JAK1A antibody produced in rabbit, Anti-JAK1B antibody produced in rabbit, Anti-JTK3 antibody produced in rabbit, Anti-Janus kinase 1 (a protein tyrosine kinase) antibody produced in rabbit

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

~130 kDa

Espèces réactives

rat, mouse, human

Concentration

1 mg/mL

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
western blot: 1:500-1:1000

Isotype

IgG

Séquence immunogène

(K-E-YP-Y-T)

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

phosphorylation (pTyr1022)

Informations sur le gène

human ... JAK1(3716)

Description générale

Janus kinase 1 (JAK1) is encoded by the gene mapped to human chromosome 1p31.3. The encoded protein is one of the four members of the mammalian JAK families. Members of JAK family contain an N-terminal FERM (four.1, ezrin, radixin, moesin) domain, through which they interact with cytokine receptors. The N-terminal end also contains an SH2-like domain. In addition, it also contains a kinase domain and a pseudo-kinase domain, which is catalytically inactive. JAK1 is ubiquitously expressed. When expressed without their receptors, JAKs reside in the cytosol. However, under normal cell conditions, they localize to plasma membrane and endosomes.

Immunogène

Peptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T), according to the protein JAK1.

Application

Anti-phospho-JAK1 (pTyr1022) antibody produced in rabbit has been used in Western blot analysis.

Actions biochimiques/physiologiques

Janus kinases (JAKs) are activated when cytokines bind with their receptors, upon which they phosphorylate the receptors and produce docking sites for signaling molecules. One of the main signaling molecules includes signal transducer and activator of transcription (Stat) family. JAK1 is needed by multiple cytokines, including the family using the shared receptor subunit called common γ chain (γc), such as interleukin (IL)-2, IL-4, IL-7, IL-9, IL-15 and IL-21. JAK1 KO (knockout) mice have early postnatal lethal or perinatal lethal phenotype due to neurological defects. In vitro studies show that JAK1S703I mutation functions as an activator of JAK-STAT pathways even in the absence of cytokines, and drives cellular proliferation. JAK1S703I mutant patient-derived xenograft (PDX) HCC (hepatocellular carcinoma) cells also show sensitivity to JAK1/2 inhibitor, ruxolitinib.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Description de la cible

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Forme physique

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Activating JAK1 mutation may predict the sensitivity of JAK-STAT inhibition in hepatocellular carcinoma.
Yang S, et al.
Oncotarget, 7(5), 5461?5469-5461?5469 (2016)
MiR-1246 Promotes LPS-Induced Inflammatory Injury in Chondrogenic Cells ATDC5 by Targeting HNF4γ.
W Da-Peng, et al.
Cellular Physiology and Biochemistry, 43(5), 2010-2021 (2017)
The Janus kinases (Jaks).
Yamaoka K et al
Genome Biology, 5(12), 253-253 (2004)
Da-Peng Wu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(5), 2010-2021 (2017-10-23)
Osteoarthritis (OA) is a common inflammatory joint disease. miRNAs are associated with OA and functionally implicated in the pathogenesis of the disease. In the present study, we investigated the role of miR-1246 in the lipopolysaccharide (LPS)-induced inflammatory injury of ATDC5

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