P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Synonyme(s) :
Proteinase K-Agarose
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About This Item
Produits recommandés
Source biologique
microbial (T.album
T. ALBUM)
Forme
lyophilized powder
Ampleur du marquage
30-140 units per g solid
Technique(s)
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
Gonflement
1 g swells to ~5 mL
Température de stockage
−20°C
Application
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Actions biochimiques/physiologiques
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Caractéristiques et avantages
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Définition de l'unité
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Forme physique
Lyophilized powder stabilized with lactose
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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