P5381

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

• Synonyme(s) : D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
• Numéro CAS: 9001-41-6
• Numéro de classification (Commission des enzymes): 5.3.1.9 (BRENDA, IUBMB)
• Numéro CE : 232-603-1
• Numéro MDL: MFCD00131887
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bakers yeast
• Type: Type III
• Forme: ammonium sulfate suspension
• Activité spécifique: ≥400 units/mg protein (biuret)
• Poids mol.: tetramer 119,500 Da±600
• Concentration: ≥3 mg/mL protein Biuret
• Technique(s): activity assay: suitable
• Couleur: white
• Numéro d'accès Protein ID: NP_009755.1
• Numéro d'accès UniProt: P12709
• Température de stockage: 2-8°C
• Informations sur le gène: yeast ... PGI1(852495)

Description

Description générale

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

Application

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:

• to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
• as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
• to convert fructose to glucose while evaluating fructose content in kudzu roots.

Actions biochimiques/physiologiques

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Définition de l'unité

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

Forme physique

Crystalline suspension in 2.6 M (NH₄)₂SO₄ containing 0.1 mM magnesium acetate, pH 7.0

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves