To reconstitute dissolve G6378, Glucose-6-phosphate Dehydrogenase, in water and a dilute buffer. Following reconstitution, it should be refrigerated at 4°C, with stock solutions remaining stable for up to 6 months at this temperature.
G6378
Glucose-6-phosphate déshydrogénase from baker′s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Synonyme(s) :
G-6-P-DH, Zwischenferment
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About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Type
Type XV
Niveau de qualité
Forme
lyophilized powder
Activité spécifique
200-400 units/mg protein (modified Warburg-Christian)
Poids mol.
128 kDa
Produit purifié par
crystallization
Application(s)
diagnostic assay manufacturing
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Catégories apparentées
Description générale
Application
La glucose-6-phosphate déshydrogénase permet de doser l'activité cétose réductase dans l'endosperme du maïs en développement.[3]
Actions biochimiques/physiologiques
La glucose-6-phosphate déshydrogénase catalyse la transformation du glucose-6-phosphate en 6-phosphogluconolacétone, ce qui constitue la première étape de la voie des pentoses phosphates<<<17>>>.
La glucose-6-phosphate déshydrogénase catalyse la transformation du glucose-6-phosphate en 6-phosphogluconolacétone, ce qui constitue la première étape de la voie des pentoses phosphates.
Définition de l'unité
Une unité oxyde 1,0 μmol de D-glucose-6-phosphate en 6-phospho-D-gluconate par minute en présence de NADP, à pH 7,4 et à 25 °C.
Forme physique
Produit lyophilisé quasiment dépourvu de sulfates, contenant environ 20 % de citrate de sodium en poids sec.
Notes préparatoires
Préparé à partir du produit réf. G7877
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Lot/Batch Number
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Les clients ont également consulté
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Sean Rogers et al.
eLife, 10 (2021-04-08)
Eukaryotes compartmentalize metabolic pathways into sub-cellular domains, but the role of inter-organelle contacts in organizing metabolic reactions remains poorly understood. Here, we show that in response to acute glucose restriction (AGR) yeast undergo metabolic remodeling of their mevalonate pathway that
Protocoles
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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What are the recommended reconstitution and storage procedures for item G6378, Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)?
1 answer-
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What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).
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What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?
1 answer-
The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.
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