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P0091

Sigma-Aldrich

PolyFreeze

water-soluble support matrix

Synonyme(s) :

water-soluble support matrix for frozen sectioning

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About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Niveau de qualité

Couleur

colorless

Densité

1.030 g/cm3

Application(s)

hematology
histology

Température de stockage

room temp

Description générale

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C. Snap freeze using PolyFreeze with isopentane and liquid nitrogen, or dry ice (slush/slurry or bunker). Store specimens frozen in PolyFreeze in liquid nitrogen canisters or in airtight containers in a -80 °C freezer. PolyFreeze does not cause autofluorescence and can easily be washed away during fixation and rinsed prior to staining. Once rinsed, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

Application

PolyFreeze has been used as an embedding medium for frozen sectioning of plant and animal samples.

Notes préparatoires

PolyFreeze is supplied ready-to-use. A cloudy appearance of the unfrozen product has no effect on its performance.

Stockage et stabilité

Store the product at room temperature

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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