This product is provided as a clear, viscous solution. Refrigeration may cause it to become difficult to use for coverslipping. Its product Information states, "Do not refrigerate". This applies to storage after the product has been opened.
F4680
Milieu de montage aqueux Fluoromount™
liquid
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A propos de cet article
UNSPSC Code:
12171500
NACRES:
NA.47
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Laissez-nous vous aiderNom du produit
Milieu de montage aqueux Fluoromount™, for use with fluorescent dye-stained tissues
Quality Segment
form
liquid
technique(s)
microbe id | staining: suitable
color
clear
suitability
suitable for fluorescence, suitable for immunohistochemistry
application(s)
diagnostic assay manufacturing
hematology
histology
General description
Fluoromount est un milieu de montage aqueux analogue au milieu Gel Mount, utilisé avec les lamelles couvre-objets pour préserver les tissus colorés à l'aide de colorants fluorescents. Ce produit est compatible avec le marquage à l'isothiocyanate de fluorescéine (FITC), la phycoérythrine, la phycocyanine et l'allophycocyanine. Il peut aussi être utilisé avec d'autres marqueurs fluorescents, comme le Texas Red et la rhodamine. Après usage, le milieu peut être facilement éliminé par immersion des lames dans de l'eau déionisée.
Application
Le milieu de montage aqueux Fluoromount™ a été utilisé pour :
- le montage de lamelles couvre-objets en vue d'une microscopie d'immunofluorescence de cellules HeLa et PC12
- le montage de cellules neuronales d'hippocampe en vue d'une observation au microscope confocal
- le montage de coupes de cerveau en vue d'une coloration par immunofluorescence[1]
Legal Information
Fluoromount is a trademark of Diagnostic BioSystems, Inc.
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Cet article | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) immunofluorescence: suitable | technique(s) - | technique(s) thin layer chromatography (TLC): suitable |
| suitability suitable for fluorescence, suitable for immunohistochemistry | suitability - | suitability - | suitability - |
| color clear | color - | color - | color - |
| form liquid | form liquid | form liquid | form liquid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can you explain why it's recommended not to refrigerate it? Does that still apply even after opening?
1 answer-
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What are some examples of aqueous mounting media without glycerol?
1 answer-
For use with fluorescence:
1. F4680 Fluoromount
2. F6182 FluoroshieldAqueous Mounting media with no glycerol and antifade agents:
1. 10981 Polyvinyl alcohol with DABCO
2. F6937 Fluoroshield with DABCO
3. F6812 Fluoroshield with PPD (not suitable for a select number of fluorescent dyes)Aqueous Mounting Media with no glycerol and another dye:
1. F6057 Fluoroshield with DAPI
2. F5932 Fluoroshield with PIAqueous Mounting Media suitable for use with light microscopy:
1.GG1 Glycerol gelatin
2.C9368 CC/MountHelpful?
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Is F4680 suitable for use as a mounting medium for AEC red IHC mounting?
1 answer-
Fluoromount is not intended for use with bright field microscopy or for coverslipping slides stained with AEC. Most fluorescent mounting media are somewhat alkaline, with a pH around 9, while AEC is typically formulated with an acetate buffer around pH 5.2. The difference in pH levels suggests that the AEC may fade quickly, especially if the slides are counterstained with a nuclear counterstain such as Mayer's Hematoxylin, which would also likely disappear rapidly.
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Does this (Fluoromount F4680) contain DAPI?
1 answer-
This product does not contain DAPI. Should DAPI be preferred, see product F6057.
F4680 Fluoromount datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/692/371/f4680pis.pdfF6057 Fluoroshield with DAPI product page:
https://www.sigmaaldrich.com/product/sigma/f6057Helpful?
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Does Fluoromount mounting media (Product No. F4680) contain an antifade reagent?
1 answer-
Fluoromount does not contain an antifade reagent. If you need an antifade mounting media, check Fluoroshield (Product Nos. F6182, F6057 and F5932) or PVA with antifade (Product Nos. 10981 and 10979).
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How do you remove the coverslip after using Fluoromount (Product No. F4680)?
1 answer-
Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does Product F4680, Fluoromount Mounting Medium, compare to GelMount (Product No. G0918) which has been deleted?
1 answer-
Product No. F4608, Fluoromount is essential the same as GelMount. GelMount was deleted by the supplier. Fluoromount is being made by another supplier with the same formulation.
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Does Fluoromount mounting media (Product No. F4680) contain glycerol?
1 answer-
Fluoromount does not contain glycerol. The exact formulation is considered proprietary.
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