Accéder au contenu
Merck
Toutes les photos(1)

Documents

M9163

Sigma-Aldrich

Medium 199

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Synonyme(s) :

M199 Medium, TCM 199

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Medium 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Niveau de qualité

Stérilité

sterile-filtered

Forme

liquid

Concentration

10 ×

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Wei Qi et al.
Journal of pharmaceutical sciences, 107(8), 2070-2078 (2018-05-02)
Stable formulations of inactivated polio vaccine (IPV) could reduce cold-chain requirements and increase distribution of the vaccine to developing countries. Recently, significant improvement in thermal stability of IPV vaccines has been achieved by including urea in lyophilized formulations. In the
Xiaofeng Yang et al.
Bioscience reports, 40(10) (2020-09-29)
Transplantion of bone marrow-derived endothelial progenitor cells (EPCs) may be a novel treatment for deep venous thrombosis (DVT). The present study probed into the role of microRNA (miR)-361-5p in EPCs and DVT recanalization. EPCs were isolated from male Sprague-Dawley (SD)
Sujoy Dutta et al.
Eukaryotic cell, 7(7), 1146-1157 (2008-05-20)
Inherent deficiencies of Leishmania in heme biosynthesis were genetically complemented for delta-aminolevulinate-inducible biosynthesis and accumulation of light-excitable uroporphyrin. The phototoxic flagellar immobilization and cytolysis phenotypes and porphyrin mobilization noted previously were further analyzed biochemically and cytologically to delineate the mechanism
Ya-Guang Liu et al.
Molecular human reproduction, 15(10), 665-673 (2009-06-10)
Transforming growth factor beta 1 (TGF-beta1) levels are increased in the peritoneal fluid of endometriosis patients, and endometrial cells express TGF-beta signaling components; however, little is known regarding the role of TGF-beta in endometriosis. Our objective was to examine the
M Colomiere et al.
British journal of cancer, 100(1), 134-144 (2008-12-18)
Epidermal growth factor receptor (EGFR) is overexpressed in ovarian carcinomas, with direct or indirect activation of EGFR able to trigger tumour growth. We demonstrate significant activation of both signal transducer and activator of transcription (STAT)3 and its upstream activator Janus

Articles

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Contenu apparenté

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique