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M0650

Sigma-Aldrich

Medium 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Synonyme(s) :

Cell growth medium, M199 Medium, TCM 199

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Medium 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Niveau de qualité

400

Stérilité

sterile-filtered

Forme

liquid

Concentration

10 ×

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Composants

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

  • to culture human mesothelial cells
  • as a component of the holding medium for the collection of embryos from pluriparous Boer goat
  • in the preparation of spheroid-embedded collagen matrices

Reconstitution

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Technical Note: Transfer of caprine blastocysts vitrified by the open pulled straw (OPS) or the solid surface procedure and warmed in sucrose-free medium
Garza D, et al.
Small Ruminant Research, 165, 111-114 (2018)
Klaus Kratochwill et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 27(3), 937-946 (2011-08-23)
Exposure of mesothelial cells to peritoneal dialysis fluids (PDF) results in cytoprotective cellular stress responses (CSR) that counteract PDF-induced damage. In this study, we tested the hypothesis that the CSR may be inadequate in relevant models of peritoneal dialysis (PD)
Elena García-Gareta et al.
BioResearch open access, 2(6), 412-420 (2014-01-01)
The clinical demand for effective dermal substitutes continues as current commercially available products present limitations. However, there are no definitive in vitro methods to predict in vivo outcomes such as integration, cellularization and contraction, which may help the development of
Ming Yu et al.
Nature metabolism, 2(10), 1013-1020 (2020-09-09)
The intrahepatic milieu is inhospitable to intraportal islet allografts1-3, limiting their applicability for the treatment of type 1 diabetes. Although the subcutaneous space represents an alternate, safe and easily accessible site for pancreatic islet transplantation, lack of neovascularization and the resulting
Fabian Tetzlaff et al.
Bio-protocol, 8(17), e2995-e2995 (2018-09-05)
Angiogenesis, the formation of new blood vessels from pre-existing ones plays an important role during organ development, regeneration and tumor progression. The spheroid-based sprouting assay is a well-established and robust method to study the influence of genetic alterations or pharmacological
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Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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