To avoid contamination, sterile filtration is highly recommended prior to cell culture application. Upon verification, the lab has not formulated a protocol for cell culture use. However, the following citation on M1753 for cell culture application may be of interest as a protocol reference: Im, H. (2011). "Mouse Embryonic Stem Cell Maintenance for Differentiation." Bio-101: e145. DOI: 10.21769/BioProtoc.145.
M1753
1-Thioglycérol
≥97%
Synonyme(s) :
α-Monothioglycérol, α-Thioglycérol, 3-Mercapto-1,2-propanediol
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About This Item
Formule linéaire :
HSCH2CH(OH)CH2OH
Numéro CAS:
Poids moléculaire :
108.16
Beilstein:
1732046
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352201
ID de substance PubChem :
Nomenclature NACRES :
NA.25
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Niveau de qualité
Essai
≥97%
Forme
liquid
Indice de réfraction
n20/D 1.527 (lit.)
pb
118 °C/5 mmHg (lit.)
Densité
1.25 g/mL at 25 °C (lit.)
Température de stockage
2-8°C
Chaîne SMILES
OCC(O)CS
InChI
1S/C3H8O2S/c4-1-3(5)2-6/h3-6H,1-2H2
Clé InChI
PJUIMOJAAPLTRJ-UHFFFAOYSA-N
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Description générale
1-Thioglycerol stimulates the synthesis of porphyrin in aerobically growing Escherichia coli. [1] It is an inhibitor of glycerol kinase activity in vitro and in situ.
Application
Notes préparatoires
This product is miscible in ethanol (1 ml/ml, 50%, v/v), yielding a clear, colorless solution. It is also miscible in water (0.1 M).
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
210.2 °F - closed cup
Point d'éclair (°C)
99 °C - closed cup
Équipement de protection individuelle
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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What could be causing contamination of cell culture experiments while using M1753? Also, how should M1753 be added to the cell culture media?
1 answer-
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What is the concentration of the 1-thioglycerol?
1 answer-
The liquid is not a solution of 1-thioglycerol in some solvent. Rather, it is a neat liquid (i.e., essentially pure 1-thioglycerol). Based on a density of 1.245 g/mL and a molecular weight of 108.2 g/mole, one can calculate an effective molarity of 11.5 M. This information may be found on the M1753 product information sheet.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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