L7261

Lipopolysaccharides from Salmonella enterica serotype typhimurium

purified by trichloroacetic acid extraction

• Synonyme(s) : LPS
• Numéro MDL: MFCD00164404
• Code UNSPSC : 12352201
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: Salmonella enterica (Serotype typhimurium)
• Niveau de qualité: 200
• Forme: lyophilized powder
• Produit purifié par: trichloroacetic acid extraction
• Impuretés: 1-10% Protein (Lowry)
• Couleur: white to faint yellow
• Solubilité: water: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

This product is TCA extracted from Salmonella typhimurium. The source strain is ATCC 7823. This LPS serotype has been used to induce NOS in rats and guinea pigs.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)