I3769
Insulin-like Growth Factor-I human
IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonyme(s) :
somatomedin c
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About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in E. coli
Pureté
≥95% (HPLC)
Forme
lyophilized powder
Puissance
0.1-10 ng/mL ED50/EC50
Qualité
endotoxin tested
Poids mol.
~7.6 kDa
Conditionnement
pkg of 50 and 100 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
≤1.00 EU/μg
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
human ... IGF1(3479) , Igf1(16000)
Description générale
Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.
Application
Insulin-like Growth Factor-I human has been used:
- in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
- in culture media, to study its effect on steroidogenesis during testicular development
- in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation
Actions biochimiques/physiologiques
IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.
Forme physique
Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.
Reconstitution
Reconstitute in water
Remarque sur l'analyse
The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Hess MF and Roser JF
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Experimental Hematology, 29(11), 1278-1288 (2001)
Juan E Puche et al.
Journal of translational medicine, 10, 224-224 (2012-11-15)
Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes. IGF-I is partly responsible for systemic GH activities
M J E Walenkamp et al.
Endocrine development, 24, 128-137 (2013-02-09)
Molecular defects of the insulin-like growth factor 1 gene (IGF1) are rare in the human. Only three homozygous and two families with heterozygous mutations of the IGF1 gene have been described, resulting in a variable degree of intrauterine and postnatal
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