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GERPN3004

ECL Detection Reagents

Cytiva RPN3004, pack of 1 ea

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About This Item

Code UNSPSC :
41105300
Nomenclature NACRES :
NA.31

Conditionnement

pack of 1 ea

Fabricant/nom de marque

Cytiva RPN3004

Température de stockage

2-8°C

Description générale

The world’s first commercially available chemiluminescent detection reagent for Western blotting. Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 minutes. It is the substrate of choice for target amounts above 500 fg.

High sensitivity and fast results:

  • Detects up to 10 to 12 ng of protein, which is ~10× more than colorimetric methods
  • Optimized to use with Amersham Protran nitrocellulose (NC) membranes
  • Delivers results in just a few minutes, then image using a CCD imager like the Amersham ImageQuant 800 or Amersham Hyperfilm ECL

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

ECL is a trademark of Cytiva

Code de la classe de stockage

12 - Non Combustible Liquids


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K Aogi et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(10), 2790-2797 (1999-10-28)
The analysis of the tissue expression patterns of both the telomerase enzyme and the adhesion molecule CD44 has highlighted these molecules as potential tumor markers. In this study, the expression of these markers was analyzed in frozen tissue samples of
G Y Akita et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 5(2), 154-158 (1993-04-01)
A previously described bluetongue virus (BTV) serogroup polymerase chain reaction (PCR) assay was applied to clinical samples. The sensitivity of the BTV serogroup PCR was increased by the use of non-radioactive chemiluminescent hybridization. Unfractionated whole blood samples from rams experimentally
S Olschwang et al.
Journal of medical genetics, 38(6), 356-360 (2001-06-05)
Germline mutations of the STK11/LKB1 tumour suppressor gene (19p13.3) are responsible for Peutz-Jeghers syndrome (PJS), a rare genetic disorder, which is dominantly inherited. In addition to the typical hamartomatous gastrointestinal polyps and perioral pigmented lesions, PJS is also associated with
Shin-Ichi Miyata et al.
Microbiology (Reading, England), 149(Pt 8), 2243-2250 (2003-08-09)
Thymidylate kinase (TMK) catalyses the phosphorylation of dTMP to form dTDP in both the de novo and salvage pathways of dTTP synthesis in both prokaryotes and eukaryotes. Two homologues of bacterial thymidylate kinase genes were identified in a genomic library
Yasuko Kimura et al.
Yeast (Chichester, England), 19(16), 1437-1445 (2002-12-13)
We recently discovered, on the chromosome of Saccharomyces cerevisiae sigma 1278b, novel MPR1 and MPR2 genes required for resistance to a toxic analogue of L-proline, L-azetidine-2-carboxylic acid. The MPR genes, which were absent in the S. cerevisiae genome project strain

Articles

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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