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Principaux documents

B6429

Sigma-Aldrich

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonyme(s) :

10x Blocking Buffer

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

for molecular biology

Niveau de qualité

200

Forme

solution

Technique(s)

Southern blotting: suitable

Température de stockage

room temp

Catégories apparentées

Description générale

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[1]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[1] to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[2][3][4]
  • enzyme linked immunosorbent assay (ELISA)[5]
  • immunohistochemistry[6]

Caractéristiques et avantages

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Notes préparatoires

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000386787
0000377173
0000368122
0000377173
0000368122

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M B Engelund et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
Afficher tous les articles scientifiques apparentés

Protocoles

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

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Questions

1–5 of 5 Questions  

  1. whats the percentage casein in 10X of B6429-500ml ?

    1 answer

    1. Unfortunately, the concentration of casein is considered proprietary.

      Helpful?

  2. Will my RNA samples and probes be safe with this buffer if we are using horseradish peroxidase and chemiluminescent detection?

    1 answer

    1. The 10X Casein Blocking Buffer is recommended for both colorimetric and chemiluminescent detection. It is used as a blocking solution for detection, which occurs after hybridization and is suitable for Northern and Southern applications.

      Helpful?

  3. What is the pH? What is the counter ion? Is there saline salt in the preparation (0.15M)?

    1 answer

    1. Unfortunately, the composition of this product is proprietary and can't be disclosed.

      Helpful?

  4. What is the % w/v casein in this solution?

    1 answer

    1. The casein concentration is 1.8% of the total solution.

      Helpful?

  5. What is the solution solvent? What grade of reagent is this in terms of sterility and associated testing?

    1 answer

    1. As mentioned in the product detail page, this product is a molecular biology-grade product. A solution is prepared by diluting the 10x Blocking Buffer with molecular biology-grade water.

      Helpful?

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