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G6148

Sigma-Aldrich

Glasgow Minimum Essential Medium

With L-glutamine, without tryptose phosphate broth and sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

BHK medium, GMEM

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About This Item

Numéro MDL:
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

Glasgow Minimum Essential Medium is suitable for the study of genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used:
  • in toxin entry assays
  • for 3 H-palmitate labeling
  • to culture embryonic stem cells

Quantité

Formulated to contain 12.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.75 g/L sodium bicarbonate and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Eye Dam. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Keiichi Ito et al.
Genes & development, 35(9-10), 729-748 (2021-04-24)
The MED1 subunit has been shown to mediate ligand-dependent binding of the Mediator coactivator complex to multiple nuclear receptors, including the adipogenic PPARγ, and to play an essential role in ectopic PPARγ-induced adipogenesis of mouse embryonic fibroblasts. However, the precise
Tatsushi Miyazaki et al.
PloS one, 8(8), e71645-e71645 (2013-08-14)
Tcl1 is highly expressed in embryonic stem (ES) cells, but its expression rapidly decreases following differentiation. To assess Tcl1's roles in ES cells, we generated Tcl1-deficient and -overexpressing mouse ES cell lines. We found that Tcl1 was neither essential nor
Naiara Clemente Tavares et al.
Frontiers in microbiology, 11, 963-963 (2020-07-01)
Schistosomiasis is a neglected tropical disease (NTD) caused by helminthes from the Schistosoma genus. This NTD can cause systemic symptoms induced by the deposition of parasite eggs in the host liver, promoting severe complications. Functional studies to increase knowledge about
Wander de Jesus Jeremias et al.
PloS one, 12(6), e0178829-e0178829 (2017-06-18)
Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the
Edward C Hsiao et al.
PloS one, 3(7), e2532-e2532 (2008-07-04)
Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter

Protocoles

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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