G5420
Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Anti-GUS
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen 60 kDa
Espèces réactives
plant
Concentration
~1.5 mg/mL
Technique(s)
western blot: 0.5-1 μg/mL using GUS purified from E. coli
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Description générale
β-Glucuronidase (GUS) is encoded by the E. coli gus gene (also referred to as uidA), GUS protein (60 kDa) is an hydrolase.
Spécificité
Anti-β-Glucuronidase (N-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.
Immunogène
Sythetic peptide corresponding to amino acids located at the N-terminus of E. coli GUS, conjugated to KLH.
Application
Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit has been used in western blotting.
Rabbit polyclonal anti-β-Glucuronidase (N-Terminal) antibody may be used for the detection of GUS by immunoblotting (60 kDa). It may be used to detect the reporter GUS marker protein in transgenic plants.
Actions biochimiques/physiologiques
β-Glucuronidase (GUS) is a screenable markers E. coli GUS has been extensively used to monitor transgene delivery to plant tissue.
β-Glucuronidase catalyzes the cleavage of terminal glucuronic acid bound by β-linkage from mono-, oligo-, or polysaccharides or phenols. GUS is mainly used as a marker during transgenic events. In vitro GUS activity assays predominantly facilitates histochemical and fluorometric localization in tissues. GUS activity is also used for the analysis of expression of foreign genes. GUS reporter genes fused with specific promoter genes have been used for the determination of transformed tissues on culture media containing antibiotics. GUS-specific antibodies are helpful in detecting GUS gene product in transformed plants.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Produit(s) apparenté(s)
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Gregory M Pellegrino et al.
Nucleic acids research, 51(2), 982-996 (2023-01-12)
The ability to restrict gene expression to a relevant bacterial species in a complex microbiome is an unsolved problem. In the context of the human microbiome, one desirable target metabolic activity are glucuronide-utilization enzymes (GUS) that are implicated in the
Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUS
Fior S, et al.
Plant methods, 5(1), 19-19 (2009)
Miroslav Krausko et al.
Plant signaling & behavior, 16(9), 1925020-1925020 (2021-06-01)
IRON-REGULATED TRANSPORTER 1 (IRT1) is a central iron transporter responsible for the uptake of iron from the rhizosphere to root epidermal cells. This study uses immunohistochemistry, histochemistry, and fluorometry to show that this gene's promoter is also active in the
GUS and GFP transformation of the biocontrol strain Clonostachys rosea IK726 and the use of these marker genes in ecological studies
Lubeck M, et al.
Mycological research, 106(7), 815-826 (2002)
Leelavathi Sadhu et al.
Scientific reports, 13(1), 896-896 (2023-01-18)
Chloroplasts have evolved from photosynthetic cyanobacteria-like progenitors through endosymbiosis. The chloroplasts of present-day land plants have their own transcription and translation systems that show several similarities with prokaryotic organisms. A remarkable feature of the chloroplast translation system is the use
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