L929 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011425, mouse adipose tissue, Fibroblast

• Synonyme(s) : Earles′s Cells, L Cells, NCTC clone 929 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: mouse adipose tissue
• Conditionnement: tube of 5 μg 85011425-DNA-5UG; pkg of vial of cells 85011425-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse C3H/An connective tissue

Description de la lignée cellulaire

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

Application

Virus studies: PRV, VSV (Indiana), HSV

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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