P3U1

85011417, mouse skin, Lymphoblast

• Code UNSPSC : 41106514

Propriétés

• product name: P3U1, 85011417
• Source biologique: mouse skin
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Lymphoblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse myeloma

Description de la lignée cellulaire

Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.

Application

Fusion partner, hybridisation and tumourigenicity studies

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.

Autres remarques

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