C9301

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

• Numéro CAS: 9007-34-5
• Numéro CE : 232-697-4
• Numéro MDL: MFCD00130825
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.75

Propriétés

• product name: Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
• Source biologique: chicken (Sternal cartilage)
• Niveau de qualité: 300
• Type: Type II (Miller)
• Gamme de produits: BioReagent
• Forme: powder
• Conditionnement: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• Concentration: 60-80% (biuret)
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 6‑10 μg/cm²
• Solubilité: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• Numéro d'accès UniProt: P02460
• Spécificité de la liaison: Peptide Source: Fibrinogen
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: chicken ... COL2A1(395069)

Description

Description générale

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

Actions biochimiques/physiologiques

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Caractéristiques et avantages

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Autres remarques

Major component of articular cartilage.

Notes préparatoires

Prepared by a modification of the method of Trentham, D.E., et al.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)