C7774
Human Collagen Type I
from human placenta, powder, suitable for cell culture
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.61
Produits recommandés
product name
Collagène from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
Source biologique
human placenta
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Impuretés
HIV, hepatitis B and hepatitis C, none detected
Température de stockage
2-8°C
Informations sur le gène
bovine ... CSN2(281099) , CSN3(281728)
human ... COL4A3(1285) , COL4A4(1286) , COL4A6(1288)
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Application
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Actions biochimiques/physiologiques
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Composants
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Notes préparatoires
Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
Autres remarques
Le collagène se classe en un certain nombre de types structurellement et génétiquement distincts. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre les désignations utilisées par Sigma et les types de classification reconnus.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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