56575
GenElute™-LPA
Neutral carrier for ethanol precipitation
Synonyme(s) :
linear polyacrylamide
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52
Produits recommandés
Niveau de qualité
Température de stockage
2-8°C
Application
GenElute™-LPA has been used to:
- extract DNA from cyanobacterial and algal cultures
- precipitate genomic DNA
- extract and purify DNA from snap-frozen uveal melanoma tissues
- to extract RNA from isolated CD4+ cells
Actions biochimiques/physiologiques
The presence of LPA during ethanol precipitation results in complete recovery of fragments larger than 20 base pairs, whereas most of the DNA is lost if no carrier is used. Very short DNA fragments, less than 20 base pairs, do not coprecipitate with LPA, allowing separation of labeled DNA from unreacted nucleotides by precipitation after the labeling reaction.
Conditionnement
GenElute LPA is tested for nucleases, and is supplied in nuclease-free water as a 25 mg/ml solution.
Principe
GenElute LPA Linear Polyacrylamide is an efficient neutral carrier for precipitating picogram amounts of nucleic acids with ethanol. The nucleic acid precipitate can be collected simply by centrifugation. LPA offers several advantages for recovering DNA or studying DNA-protein interactions, relative to other carriers, such as tRNA or glycogen. tRNA interferes with DNA during phosphorylation with polynucleotide kinase and glycogen competes with protein in DNA-protein interaction studies. In contrast, LPA is completely inert. LPA is synthesized chemically and, therefore, is not contaminated with biological material. The precipitate is visible immediately upon addition of LPA, thus eliminating wait time and low temperature incubation.
Informations légales
GenElute is a trademark of Sigma-Aldrich Co. LLC
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Quantitative PCR enumeration of total/toxic Planktothrix rubescens and total cyanobacteria in preserved DNA isolated from lake sediments.
Savichtcheva O
Applied and Environmental Microbiology, 77(24), 8744-8753 (2011)
Whole-genome microarray detects deletions and loss of heterozygosity of chromosome 3 occurring exclusively in metastasizing uveal melanoma.
Lake SL
Investigative Ophthalmology & Visual Science, 51(10), 4884-4891 (2010)
Promoter de-methylation of cyclin D2 by sulforaphane in prostate cancer cells.
Hsu A
Clinical epigenetics, 3, 3-3 (2011)
Ethanol precipitation of DNA with linear polyacrylamide as carrier.
C Gaillard et al.
Nucleic acids research, 18(2), 378-378 (1990-01-25)
Peripheral immune responses in pregnant cattle following Neospora caninum infection.
Rosbottom A
Parasite Immunology, 29(4), 219-228 (2007)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique