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NA1111

Sigma-Aldrich

GenElute™ Gel Extraction Kit

sufficient for 70 purifications

Synonyme(s) :

DNA gel extraction kit, DNA gel isolation kit, DNA gel purification kit, PCR gel clean up kit, PCR gel isolation kit, gel isolation kit, gel purification kit, Rapid purification of linear DNA

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 70 purifications

Technique(s)

DNA purification: suitable

Température de stockage

15-25°C

Description générale

The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.

Application

GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.
The isolated DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Caractéristiques et avantages

  • Bind up to 10 μg of DNA
  • Recoveries up to 80%
  • Up to 3.5 g can be processed per column
  • Compatible with both standard and low-melting agarose in TAE or TBE buffer

Principe

The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted.

The purified DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, and labeling.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

En option

Réf. du produit
Description
Tarif

Pictogrammes

CorrosionExclamation mark
GHS05,GHS07

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - STOT SE 3

Organes cibles

Respiratory system

Risques supp

EUH032

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Karen Olsson-Francis et al.
Applied and environmental microbiology, 76(7), 2115-2121 (2010-02-16)
Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria
Prolactin inhibits a major tumor-suppressive function of wild type BRCA1.
Chen KH and Walker Am
Cancer Letters, 375(2), 293-302 (2016)
Charles S Cockell et al.
The ISME journal, 5(10), 1671-1682 (2011-05-20)
An epilithic microbial community was launched into low Earth orbit, and exposed to conditions in outer space for 548 days on the European Space Agency EXPOSE-E facility outside the International Space Station. The natural phototroph biofilm was augmented with akinetes
Cassandra M Barrett et al.
International journal of molecular sciences, 21(2) (2020-01-18)
A persistent challenge for mammalian cell engineering is the undesirable epigenetic silencing of transgenes. Foreign DNA can be incorporated into closed chromatin before and after it has been integrated into a host cell's genome. To identify elements that mitigate epigenetic
Vernon K Ward et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(26), 11050-11055 (2007-06-22)
Noroviruses are the major cause of nonbacterial gastroenteritis in humans. These viruses have remained refractory to detailed molecular studies because of the lack of a reverse genetics system coupled to a permissive cell line for targeted genetic manipulation. There is
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Protocoles

GenElute™ Gel Extraction procedure

The GenElute Gel Extraction kit is designed for the rapid purification of 50 bp to 10 kb linear DNA fragments and plasmids from standard or low-melting agarose gels.

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