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3023

Sigma-Aldrich

AptaFluor® SAH Methyltransferase Assay

Synonyme(s) :

Methyltransferase Activity Assay

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About This Item

Code UNSPSC :
41122100
Nomenclature NACRES :
NA.84

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

The AptaFluor® SAH Methyltransferase TR-FRET Assay is a universal assay for enzymes that convert S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH), with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The assay can be used for all enzymes within the histone methyltransferase (HMT) and DNA methyltransferase (DNMT) families. The assay is designed specifically for high-throughput screening (HTS), with a simple mix-and-read format and 2 liquid addition steps. It is run in an endpoint mode: methyltransferase (MT) enzyme reactions are quenched with a universal stop reagent (provided) and then the detection reagents are added.

The assay offers reagent stability and compatibility with commonly used multimode plate readers. The AptaFluor® SAH Methyltransferase TR-FRET Assay provides the following benefits:
  • Simple, mix-and-read format ideal for HTS.
  • Robust MT detection under initial velocity conditions (=20% conversion of SAM to SAH).
  • Accommodates SAM concentrations as low as 100 nM and as high as 5 μM.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

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Quantité

3023-B = 100 assay, 96-well
3023-1K = 1,000 assay, 384-well

Forme physique

Kit with buffered aqueous solutions

Informations légales

AptaFluor is a registered trademark of BellBrook Labs
Transcreener is a registered trademark of BellBrook Labs

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L Latini et al.
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Autophagy is the evolutionarily conserved degradation and recycling of cellular constituents. In mammals, autophagy is implicated in the pathogenesis of many neurodegenerative diseases. However, its involvement in acute brain damage is unknown. This study addresses the function of autophagy in
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The Journal of cell biology, 208(7), 975-986 (2015-03-25)
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