03978

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

• Synonyme(s) : Loeffler’s Methylene Blue
• Formule empirique (notation de Hill): C₁₆H₁₈ClN₃S
• Numéro CAS: 61-73-4
• Poids moléculaire : 319.85
• Numéro Beilstein : 3641570
• Numéro MDL: MFCD00012111
• Code UNSPSC : 12171500
• ID de substance PubChem : 329747853
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy; concentrated, aqueous solution
• Forme: liquid
• Durée de conservation: ~3 yr
• Concentration: 1.5% (1.5 g/ 100 mL)
• Densité: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• Clé InChI: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

Description générale

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter