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341088

Sigma-Aldrich

1,9-Dimethyl-Methylene Blue zinc chloride double salt

Dye content 80 %

Synonyme(s) :

DMMB, Taylor’s Blue

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About This Item

Formule empirique (notation de Hill):
C18H22ClN3S · 0.5ZnCl2
Numéro CAS:
931418-92-7
Poids moléculaire :
416.05
Numéro MDL:
MFCD19686959
Code UNSPSC :
12171500
ID de substance PubChem :
24860900
Nomenclature NACRES :
NA.47

Forme

powder

Niveau de qualité

200

Composition

Dye content, 80%

Impuretés

<20% water

Pf

250 °C (dec.) (lit.)

λmax

649 nm

ε (coefficient d'extinction)

≥10000 at 243-249 nm in H2O
≥18000 at 285-291 nm in H2O
≥25000 at 594-600 nm in H2O
≥35000 at 646-652 nm in H2O

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

[Cl-].[Cl-].Cl[Zn]Cl.CN(C)c1cc(C)c2N=C3C(C)=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4cc(C)c5N=C6C(C)=C\C(C=C6Sc5c4)=[N+](/C)C

InChI

1S/2C18H22N3S.4ClH.Zn/c2*1-11-7-13(20(3)4)9-15-17(11)19-18-12(2)8-14(21(5)6)10-16(18)22-15;;;;;/h2*7-10H,1-6H3;4*1H;/q2*+1;;;;;+2/p-4

Clé InChI

MKLBAKXCEQNMBC-UHFFFAOYSA-J

Application

1,9-Dimethyl-Methylene Blue zinc chloride double salt has been used for the quantification of sulfated glycosaminoglycans.
DMMB is a cationic dye that specifically binds to sulfated glycosaminoglycans (sGAG) with an absorbance at ~525 nm. It has been used to quantitate glycosaminoglyans such as chondroitin sulfate, keratin sulfate, and heparan sulfate and sGAG chains in proteoglycans. One limitation to this method The sGAG-DMMB complex is unstable in solution and may precipitate. Precipitation of the complex was shown to improve stability.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Conseils de prudence

P305 + P351 + P338

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000241219
MKCM5250
MKCH6422
MKCF2084
MKCF2085

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Consulter la Bibliothèque de documents

Hyeon-Son Choi et al.
Biological & pharmaceutical bulletin, 39(12), 2042-2051 (2016-12-03)
The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1β-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion
A Herrero-Mendez et al.
Journal of tissue engineering and regenerative medicine, 10(12), 1000-1011 (2014-03-07)
Biomaterials and, especially, scaffolds may function as temporary extracellular matrix (ECM), mimicking in vivo environmental structures and facilitating cell growth and tissue regeneration. ECM is composed mostly of glycosaminoglycans (GAGs) and proteins, the ratio of GAGs, hyaluronic acid (HA):sulphated GAGs
Tianyi Wang et al.
Tissue engineering. Part A, 22(23-24), 1348-1356 (2016-10-21)
Cell-based therapies offer great promise for repairing cartilage. Previous strategies often involved using a single cell population such as stem cells or chondrocytes. A mixed cell population may offer an alternative strategy for cartilage regeneration while overcoming donor scarcity. We
Makwese Maepa et al.
Tissue engineering. Part A, 25(15-16), 1137-1145 (2018-12-05)
Eucomis autumnalis is one plant that is used by various traditional healers to alleviate the signs and symptoms associated with osteoarthritis. Although the exact mechanisms remain unknown, we hypothesized that this plant can induce chondrogenesis. In this work, we explored
Anita Krouwels et al.
Spine, 43(5), 307-315 (2015-04-10)
An in vitro study using human degenerated nucleus pulposus cells. To determine the effect of osmolality and different osmolytes on the regeneration by human nucleus pulposus cells through gene expression and extracellular matrix production. Intervertebral disc (IVD) degeneration is a
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