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10127655001

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

Synonyme(s) :

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

Numéro de classification (Commission des enzymes):
1.1.1.49 (BRENDA, IUBMB)
Code UNSPSC :
12352204

Source biologique

yeast

Niveau de qualité

100

Forme

suspension

Activité spécifique

~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)

Conditionnement

pkg of 1 mL (5 mg/ml)

Fabricant/nom de marque

Roche

Concentration

≥0.1-1.0 % (w/w)

Technique(s)

activity assay: suitable

Couleur

white to yellowish

pH optimal

9.2

Solubilité

water: miscible

Adéquation

suitable for UV spectrophotometry and general use

Numéro d'accès NCBI

CAA93357

Numéro d'accès UniProt

P11412

Application(s)

life science and biopharma

Activité étrangère

6-PGDH <0.0100%
CK <0.00100%
GR <0.0100%
HK <0.0100%
PGI <0.00200%
PGluM <0.0100%

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

Saccharomyces cerevisiae ... ZWF1(855480)

Catégories apparentées

Description générale

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP+ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP+ and NADPH levels the equilibrium shifts to inactive tetramers.

Application

Component of cofactor recycling systems for NADPH.

Actions biochimiques/physiologiques

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP+ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Caractéristiques

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI
Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Activator: Mg2+ (5 to 10 mM)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Matthew G S Norris et al.
Biochemical and biophysical research communications, 405(3), 388-392 (2011-01-18)
Enzyme kinetic parameters for rate equations are vital in metabolic network simulation, a major part of systems biology research efforts. Measurements of Michaelis-Menten kinetic parameters Km and Kcat have been performed for enzymes glucose-6-phosphate dehydrogenase (G6P DH) under crowded conditions
Chien-I Yang et al.
iScience, 25(8), 104756-104756 (2022-08-10)
The removal of the N-terminal formyl group on nascent proteins by peptide deformylase (PDF) is the most prevalent protein modification in bacteria. PDF is a critical target of antibiotic development; however, its role in bacterial physiology remains a long-standing question.
Bárbara Della Noce et al.
The Journal of biological chemistry, 298(3), 101599-101599 (2022-01-23)
Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role in maintaining physiological homeostasis and in preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance
Neal J Dawson et al.
eLife, 9 (2020-07-31)
High-altitude environments require that animals meet the metabolic O2 demands for locomotion and thermogenesis in O2-thin air, but the degree to which convergent metabolic changes have arisen across independent high-altitude lineages or the speed at which such changes arise is

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